Champlain allied New France with which First Nations, historic enemies of the Iroquois?

Answer: The Algonquin, Montagnais and Huron

Champlain allied New France with the Algonquin, Montagnais and Huron, who were old enemies of the Iroquois. The Iroquois, a confederation of five (later six) First Nations, fought the French settlements for about a century until peace was made in 1701.

Learn more: Royal New France

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